Aldehyde dehydrogenase 2 rs671 genetic polymorphisms are associated with chemotherapy-induced nausea and vomiting / 南方医科大学学报

OBJECTIVE@#To investigate the correlation between aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphisms and chemotherapy-induced nausea and vomiting (CINV).@*METHODS@#A total of 90 Chinese patients with malignant tumors receiving chemotherapy for the first time were recruited in this study. The occurrence of CINV was observed within 120 h after treatment with docetaxel and cis-platinum chemotherapy (DP regimen). The data of the patients (including age, gender, tumor stage, habitual alcohol consumption, motion sickness, morning sickness, and average sleep time prior to chemotherapy) were collected through a questionnaire. ALDH2 rs671 polymorphisms of the patients were analyzed using a multiple single nucleotide polymorphism genotyping, and the Hardy-Weinberg equation was used for genetic linkage analysis. The correlations between the factors including ALDH2 rs671 polymorphisms and the occurrence of CINV were analyzed.@*RESULTS@#The incidence of CINV was 48.9% among the patients receiving their first chemotherapy with DP regimen. Univariate analysis indicated that the genetic polymorphisms of ALDH2 rs671 were significantly correlated with the occurrence of CINV (P < 0.05). Multivariate logistic analysis indicated that ALDH2 rs671 mutation (OR: 3.019, 95% CI: 1.056-8.628, P < 0.05) and average sleep time prior to chemotherapy no longer than 6 h (OR: 2.807, 95% CI: 1.033-7.628, P < 0.05) were risk factors for CINV in patients with malignant tumors receiving the first chemotherapy with DP regimen.@*CONCLUSION@#ALDH2 gene mutation at rs671 is a risk factor contributing to the occurrence of CINV, and understanding of the underlying mechanism may help to more effectively control the occurrence of CINV.

Management of liver transplantation perioperative period in acute-on-chronic liver failure / 中华肝脏病杂志

Acute-on-chronic liver failure (ACLF) is a potentially reversible entity that occurs in patients with chronic liver disease accompanied with or without cirrhosis and is characterized by extrahepatic organ failure and high short-term mortality. Currently, the most effective treatment method for patients with ACLF is liver transplantation; therefore, admission timing and contraindications must be emphasized. The function of vital organs such as the heart, brain, lungs, and kidneys should be actively supported and protected during the liver transplantation perioperative period in patients with ACLF. Focusing on the anesthesia management level during anesthesia selection, intraoperative monitoring, three-stage management, prevention and treatment of post-perfusion syndrome, monitoring and management of coagulation function, volume monitoring and management, and body temperature monitoring management for liver transplantation should strengthen anesthesia management. Additionally, standard postoperative intensive care treatment should be recommended, and grafts and other vital organ functions should be monitored throughout the perioperative period to promote early postoperative recovery in patients with ACLF.

Burned-out testicular germ cell tumors: a clinicopathological analysis of three cases / 中华病理学杂志

Objective: To investigate the clinicopathological features and possible mechanisms of burned-out testicular germ cell tumors. Methods: The clinical and imaging data, histology and immunophenotypic characteristics of three cases of burned-out testicular germ cell tumors diagnosed at the Ruijin Hospital, Medical College of the Shanghai Jiaotong University, from 2016 to 2020 were retrospectively analyzed. The relevant literature was reviewed. Results: The mean age of the three patients was 32 years. Case 1 had an elevated preoperative alpha-fetoprotein level (810.18 μg/L) and underwent "radical pancreaticoduodenectomy and retroperitoneal lesion resection" for a retroperitoneal mass. Postoperative pathology showed embryonal carcinoma, which needed to exclude gonadal metastasis. Color Doppler ultrasound showed a solid mass of the right testis, with hypoechoic lesion and scattered calcification in some areas. Case 2 was a "right supraclavicular lymph node biopsy specimen." Chest X-ray showed multiple metastases in both lungs. The biopsy showed metastatic embryonic carcinoma and bilateral testicular color Doppler ultrasound revealed abnormal calcifications in the right testicle. Case 3 showed a cystic mass of the right testis with calcification and solid areas. All 3 patients underwent radical right orchiectomy. Grossly, borders of the testicular scar areas were well defined. Cross sectioning of the tumors showed a gray-brown cut surface and single focus or multiple foci of the tumor. The tumor maximum diameter was 0.6-1.5 cm. Microscopically, lymphocytes, plasma cells infiltration, tubular hyalinization, clustered vascular hyperplasia and hemosiderin laden macrophages were found in the scar. Atrophic and sclerotic seminiferous tubules, proliferation of clustered Leydig cells and small or coarse granular calcifications in seminiferous tubules were present around the scar. Seminoma and germ cell neoplasia in situ were seen in case 1, germ cell neoplasia in situ was seen in case 2 and germ cells with atypical hyperplasia were seen in case 3. Immunohistochemistry showed that embryonic carcinoma expressed SALL4, CKpan(AE1/AE3) and CD30, seminoma and germ cell tumor in situ expressed OCT3/4, SALL4 and CD117, and spermatogenic cells with atypical hyperplasia expressed CD99 and SALL4. The Ki-67 positive index was about 20%, while OCT3/4 and CD117 were both negative. Conclusions: Burned-out testicular germ cell tumors are rare. The possibility of gonad testicular metastasis should be considered first for extragonadal germ cell tumor. If fibrous scar is found in testis, it must be determined whether it is a burned-out testicular germ cell tumor. The burned-out mechanisms may be related to the microenvironment of tumor immune-mediated and local ischemic injury.

Breviscapine Ameliorates Fear Extinction and Anxiety in BALB/cJ Mice

Objective@#In this research, the influence of breviscapine on anxiety, fear elimination, and aggression and the potential mechanism was investigated. @*Methods@#Anxiety and locomotion were analyzed by elevated plus maze and open field test in mice. Bussey-Saksida Mouse Touch Screen Chambers were used to perform fear conditioning. Territorial aggression was assessed by resident intruder test. Protein levels were evaluated by Western blot. Breviscapine improved fear-extinction learning in BALB/cJ mice. @*Results@#Breviscapine at 20–100 mg/kg increased center cross number, total distance traveled, and velocity in a dose-dependent manner. On the other hand, breviscapine at 20–100 mg/kg decreased the immobility time in open field test. In addition, breviscapine at 20–100 mg/kg increased the ratio of time on the open arm, time on the distal parts of the open arm, and total distance traveled in elevated plus maze. Breviscapine at 100 mg/kg increased the average attack latency and decreased the number of attacks over the last 3 days of resident intruder test. In hippocampus, protein levels of postsynaptic density protein-95 and synaptophysin were elevated by breviscapine at these three doses. @*Conclusion@#The administration of breviscapine alleviates fear extinction, anxiety, and aggression, while increases locomotor in a dose-dependent manner, which might be associated with its influence on synaptic function.

The relationships between functional gastrointestinal diseases and psychological factors, diet and lifestyles: a network analysis / 中华内科杂志

Objective:To investigate the relationship between common functional gastrointestinal diseases symptoms with psychological factors, diet and lifestyles by using the network analysis method which has achieved great success in the field of psychology in recent years.Method:A questionnaire survey was conducted in two military units using the cluster sampling method during July 2020, and a total of 1 805 subjects were included. Functional gastrointestinal disease symptoms were evaluated with the Gastrointestinal Symptom Rating Scale (GSRS). The state, trait anxiety scale and stress response scale were used to evaluate the mental and psychological state by self-evaluation. R was used to build the network and calculate statistical parameters.Results:1 486 of the 1 805 subjects (82.3%) had experienced functional gastrointestinal diseases symptoms within 2 weeks, but most of them were mild. Network analysis shows that there was a strong interaction between digestive system symptoms with different clinical manifestations (Spearman coefficient ranges 0.31-0.56). There was a clear relationship between functional gastrointestinal symptoms and mental and psychological factors (Spearman coefficient ranges 0.16-0.27), but there was no clear interaction with diet, age, education level, body mass index, etc. Functional gastrointestinal diseases symptoms were connected with mental and psychological factors through two nodes: stress and indigestion. The stability coefficient of node strength correlation was 0.75, indicating that the network was stable.Conclusions:The current study revealed the network structure and features of functional gastrointestinal diseases symptoms with mental and psychological factors. The key linking nodes provided potential interfering target for controlling functional gastrointestinal symptoms related to mental and psychological factors.

Treatment of lumbar spinal stenosis with minimally invasive transforaminal lumbar interbody fusion in Zista channel assisted by navigation / 中华骨科杂志

Objective:To analyze the clinical outcomes of minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) in the Zista channel assisted by navigation for the treatment of lumbar spinal stenosis.Methods:The medical records of 156 patients who underwent surgery for lumbar spinal stenosis from January 2017 to January 2019 were retrospectively analyzed. The patients were divided into minimally invasive group, navigation open group and open group according to surgical method and navigation usage. 67 cases were treated with MIS-TLIF assisted by navigation in minimally invasive group. In the navigation open group, 31 cases underwent open TLIF surgery assisted by navigation, 58 cases were treated with open TLIF. The database was compared among the three groups including intraoperative blood loss, operative time, postoperative drainage, postoperative hospitalization time and complications. Evaluated the internal fixation and fusion according to CT, assessed the surgical results according to VAS, ODI and SF-36. The clinical effects were evaluated by MacNab scores at the last follow-up.Results:The amount of intraoperative blood loss in the minimally invasive group 116.39±25.88 ml was less than that in the navigation open group 293.94±61.67 ml and the open group 396.97±92.58 ml, and the difference was statistically significant ( F=296.01, P<0.001). The intraoperative blood loss in the navigation open group was less than that in the open group. The postoperative drainage in the minimally invasive group 80.55±27.29 ml, was less than that in the navigation open group 299.94±50.32 ml and the open group 304.86±84.34 ml, and the difference was statistically significant ( F=273.14, P<0.001). The postoperative hospitalization time in the minimally invasive group 3.42±1.00 d was less than that in the navigation open group 7.16±1.39 d and the open group 7.31±1.69 d, and the difference was statistically significant ( F=154.49, P<0.001). There was no significant difference in the operation time ( F=0.15, P=0.859). At 2 weeks and 3 months after operation, the VAS score of low back pain in the open navigation group (3.84±0.82, 1.90±0.91) and the open group (3.67±0.92, 1.91±0.90) and ODI in the navigation open group (34.74%±11.66%, 28.68%±8.19%) and the open group (32.21%±10.66%, 27.17%±9.59%) were lower than those in the minimally invasive group (1.70±0.92, 0.96±0.73), (18.33%±7.43%, 19.15%±7.96%), and the difference were statistically significant [( F=96.63, P<0.001; F=25.12, P<0.001), ( F=45.59, P<0.001; F=18.99, P<0.001)]. The SF-36 score of the minimally invasive group 61.48±9.50 at the last follow-up was higher than that of the navigation open group 52.51±6.99 and the open group 53.48±7.66, and the difference was statistically significant ( F=18.97, P<0.001). In the same group, the VAS score, ODI score and SF-36 score at each follow-up time after surgery were statistical differences compared with those before surgery ( P<0.05). Postoperative follow-up CT showed that the fusion rate of the minimally invasive group was 94.0% (63/67), the navigation open group was 93.5% (29/31), the open group was 93.1% (54/58), and the difference between the three groups was not statistically significant (χ 2=0.05, P=0.978). The success rate and accuracy of one-time nail placement in the minimally invasive group and the navigation open group were higher than those in the open group, the difference was statistically significant (χ 2=17.17, P<0.001; χ 2=15.49, P=0.040). Dural rupture occurred in 1 patient in the minimally invasive group and 2 patients in the open group. The drainage and condition changes were closely observed after surgery. All patients were successfully extubated after surgery without complaining of other discomfort. One patient in the minimally invasive group had endplate destruction and mild intervertebral collapse during postoperative follow-up. There was 1 case of incisional fat liquefaction in each of the navigation open group and the open group. Subgroup analysis of the results of the three groups were roughly the same as the overall results. Conclusion:MIS-TLIF in the Zista channel assisted by navigation is a safe, effective, and worthy of promotion minimally invasive lumbar fusion surgery with the advantages of less trauma and faster recovery in the treatment of different types of lumbar spinal stenosis.

Effect of forgetting curve based self-management on cognitive function， daily living ability and treatment efficacy of patients with mild cognitive impairment / 四川精神卫生

ObjectiveTo discuss the effect of forgetting curve based self-management on cognitive function， daily living ability and treatment efficacy of patients with mild cognitive impairment （MCI）. MethodsSimple random sampling method was adopted to enroll 162 MCI patients who met the diagnostic criteria of "Expert Consensus on the Prevention and Treatment of Cognitive Impairment in China" in Nanchong Physical and Mental Hospital and Gaoping Ledele Seniors-Oriented Apartment from April 2020 to June 2021. The selected individuals were classified into study group and control group according to random number table methods， each with 81 cases. Both groups received routine intervention， based on this， study group received the forgetting curve based self-management. The interventions lasted for 3 months in two groups， and patients were assessed using Montreal Cognitive Assessment Scale （MoCA） and Activity of Daily Living Scale （ADL） at the baseline and end of interventions. Then the clinical efficacy was compared between groups. ResultsAfter intervention， an increase was found in MoCA and ADL scores in both groups （tcontrol group=25.004， 12.503， tstudy group=48.211， 24.949， P<0.01）， and post-intervention MoCA and ADL scores in study group were higher than those in control group （t=28.527， 9.433， P<0.01）. The overall efficacy rate was 86.42% in control group， which was lower than 96.30% in study group， with statistical difference （χ²=5.004， P<0.05）. ConclusionForgetting curve based self-management may ameliorate the cognitive function and daily living ability in MCI patients， thus improving the treatment efficacy.

Effect of pulsed epidural analgesia on nursing care of patients with uterine contraction pain after cesarean section / 中国实用护理杂志

Objective:To observe the analgesic effect and nursing intervention of pulse epidural controlled analgesia on uterine contraction pain after cesarean section.Methods:A total of 100 cases of parturient with patient-controlled epidural analgesia after elective cesarean section in Central Theater General Hospital of the Chinese People's Liberation Army from January to March 2019 were selected as study subjects, and 100 cases were divided into observation group and control group, each group of 50 cases, the control group used constant speed epidural patient-controlled analgesia, the observation group used pulse epidural patient-controlled analgesia. The analgesic effect of uterine contraction pain after cesarean section was observed, at the same time, the two groups conducted a simulation test, the speed, time and infiltration area of the pumping liquid were recorded, and the Visual Analogue Scale (VAS) scores of incision pain and uterine contraction pain were recorded at 6 h, 12 h, 24 h and 48 h postoperatively. VAS score of contraction pain after single press, Motor Block score of iatrogenic uterine pain were recorded on the postoperative day, the first day and the second day after operation.The times of additional dose compression by analgesia pump, the times of incomplete analgesia intervention, nausea and vomiting, skin itching and other adverse reactions were recorded within 2 days after operation.Results:The infiltration area at 1, 5 min were (130.00±14.14), (334.00±2.83) cm 2 in the observation group and (65.00±7.07), (137.50±3.54) cm 2 in the control group, there were significant differences between the two groups( t values were 5.814, 61.376, P< 0.05 or 0.01). VAS scores of incision pain at 6 h and 12 h after operation were (2.36±1.05) and (2.42±0.95) in the observation group and (3.52±1.09) and (3.16±1.25) in the control group, respectively, there were significant differences between the two groups( t values were 5.425, 3.331, P<0.01). VAS scores of uterine contraction pain at 6,12 and 24 hours after operation were (2.66±0.80), (2.23±0.68), (2.22±0.71) in the observation group and (5.14±1.05), (4.48±0.71), (3.36±0.80) in the control group, respectively, there were significant differences between the two groups( t values were 2.489, 2.008, 5.004, P<0.01 or 0.05).VAS scores of contraction pain after single press on the postoperative day, the first day and the second day after operation in the observation group were (2.66±0.80), (2.23±0.68), (2.22±0.71) in the observation group and (5.14±1.05), (4.48±0.71), (3.36±0.80) in the control group, respectively, there were significant differences between the two groups( t values were 13.296,15.536, 7.534, P<0.01). The times of pressing and intervention were (2.28±1.36) and (0.90±0.61) in the observation group and (4.62±1.61), (0.62±0.53) in the control group, respectively, there were significant differences between the two groups( t values were 7.847, 2.439, P<0.01 or 0.05). Maternal satisfaction score was (5.92±1.37) and (2.34±0.82) in the observation group and the control group, there was significant difference ( t value was 15.856, P<0.01).The incidence of adverse reactions such as lower limb numbness, nausea and vomiting and skin pruritus in the observation group were 2.00%(1/50), 4.00%(2/50) and 4.00%(2/50) respectively, which were lower than those in the control group 14.00%(7/50), 16.00%(8/50), 18.00%(9/50), the difference was statistically significant ( χ2 values were 4.759, 4.000, 5.005, P<0.05). Conclusions:The pulse epidural controlled analgesia can effectively reduce the uterine contraction pain after cesarean section, and the active nursing intervention can accelerate the postoperative recovery of parturient.

Treatment strategy for severe lepharoptosis / 中华整形外科杂志

Objective@#To establish a treatment protocol for severe blepharoptosis. This protocol helps to achieve better accuracy and more stable result.@*Methods@#Evaluate levator muscle function pre-operatively. When levator function ≤ 1 mm, frontalis suspension technique was performed. When levator function more than 1 mm, technique of levator resection, combining excision of tarsus and levator, and tarsus-levator-CFS suspension was performed accordingly until it reaches adequate correction result during the surgery.@*Results@#A total of 275 severe ptosis patients was included from January 2015 to June 2016. 52 cases (388 eyes) received levator resection. 162 cases received combining excision of tarsus and levator. 24 cases received tarsus-levator- CFS suspension. 37 cases received frontalis suspension. 326 eyes achieved adequate correction results. 62 cases were still undercorrected. The asymmetry result showed that 76 cases presented good symmetry. 142 cases presented moderate asymmetry and 57 showed severe asymmetry.@*Conclusions@#The new treatment protocol shows a satisfactory result with better accuracy and more stable correction for severe ptosis correction.

Study on prescription and process of Fritillariae Thunbergii Bulbus formula granules based on characterization of powdery / 中草药

Objective: To study the prescription and process of Fritillariae Thunbergii Bulbus (FTB) formula granules. Methods: FTB was extracted by decocting method. FTB extract powder was prepared by spray drying method. Wet-extruding granulating, extruded-rolling granulating, and one-step granulating were adopted for preparing the formula granules. A comprehensive evaluation method was based on the powder fluidity parameters such as the rest angle, the final volume reduction degree (a), the filling velocity constant (b, k), and the yield of the particles, to optimize the optimum preparation process, prescription excipients and their proportion. An HPLC method was used to determine the contents of peimine and peiminine. The chromatographic column was the Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm), and the flow phase was Acetonitrile-water-diethylamine (70:30:0.03); The volume flow was 1.0 mL/min; The column temperature was 30 ℃. Results: The synthesis score of the FTB formula granules prepared by one-step granulating method was the highest. The best prescription was the extract powder-dextrin- 95% ethanol solution (100:100:160). The particle yield was 91.3%, the rest angle was 30.73°, the value of a was 0.109 1, the value of b was 0.025 5 and the value of k was 0.030 1, the fluidity of the granules was good and the yield was high. The content of peimine was 0.305% and the content of peiminine was 0.098% in the particles by HPLC. Conclusion: In this experiment, the formulation and process of FTB formula granules were designed to met the design requirements, which could be used in the production of the technology.

Zista system for minimally invasive treatment of lumbar spinal stenosis / 中华骨科杂志

Objective To evaluate the clinical efficacy of the minimally invasive treatment with Zista system for lum-bar spinal stenosis. Methods April 2016 to October 2017, 45 patients with lumbar spinal stenosis (2 or 3 segments) were retro-spectively collected, including 21 males and 24 females. Age 50-74 years, average 62.5 ±11.2 years, which were divided into mini-invasive surgery group and open surgery group. In the mini-invasive group, 19 cases were treated with mini-invasive decompres-sion and intervertebral fusion internal fixation through Zista channel, including 14 cases with two segments and 5 cases with three segments. 26 cases were treated with traditional open surgery, 17 cases in two sections and 9 cases in three sections. The Japanese Orthopaedic Association score (JOA) and visual analogue scale (VAS) were used to assess the clinical effect postoperatively. The change of spinal canal in the mini-invasive group was assessed by lateral recess angle. Results All of the 45 operations had been completed successfully. The average operation time in mini-invasive surgery group was 240 ± 15 min (range, 230-310) for 2 seg-ments and 320 ± 15 min (range, 320-370) for 3 segments. The average operation time in open surgery group was 255 ± 15 min (range, 210-300) for 2 segments and 315±20 min (range, 330-390) for 3 segments, there was no significant difference between two groups. The intraoperative blood loss in mini-invasive surgery group was 220±25 ml (range, 190-310) for 2 segments and 340±30 ml (range, 280-410) for 3 segments. The intraoperative blood loss in open surgery group was 550 ± 25 ml (range, 500-730) for 2 segments and 840 ± 20 ml (range, 750-920) for 3 segments, the bleeding volume of the open surgery group was more than that of the mini-invasive group. All patients were followed up, and the follow-up time was 7.3±3.2 months (range, 3-12 months) in mini-invasive surgery group and 8.1 ± 2.6 months (range, 3-12 months) in open surgery group respectively. The VAS score of low backpain and lower limb pain in the two groups was decreased 4.2 and 5.4 in mini-invasive group, 4.7 and 5.1 in open surgery group at 3 months after operation. The average JOA score of the two groups increased 13.3 in mini-invasive group and 12.7 in open surgery group at 3 months after the operation. The latera recess angle in mini-invasive group between preoperation and postoperation was significantly different. No incisional infection, implant loosening, discitis and leakage of cerebrospinal fluid were found. Conclu-sion Mini-invasive decompression and internal fixation through Zista channel for lumbar spinal stenosis is a safe, effective and minimally invasive method of operation with the characteristics of short operation time, less intraoperative blood loss and low com-plications.

Physiological strategy for treatment of mild and moderate upper eyelid with ptosis / 中华医学美学美容杂志

Objective Patients with mild or moderate blepharoptosis performed with double eyelid operation only will lead to high complication rates,such as eyelid crease disappearance,unphysiological appearance.In such cases,utilizing levator aponeurosis can create more physiological double eyelid and correct blepharoptosis in the meantime.Methods Levator aponeurosis is utilized as "connecting tissue" to transmit dynamic "motor power" of levator muscle to upper eyelid.Levator advancement was performed at the same time to correct blepharoptosis.Results A total of 74 patients (125 eyes) were operated on in this study.117 eyes (93.6％) showed adequate blepharoptosis correction results,8 eyes (6.4％) showed inadequate correction results.Sixty six cases (89.2％) showed good symmetry results,5 cases (6.8％) showed fair results,3 cases (4％) showed bad symmetry results.One hundred and fourteen eyes (91.2％) showed stable crease,11 eyes (8.8％) showed fair results,no disappearance result was observed in this study.No scar hypertrophy or blepharoptosis on the upper eyelid was encountered.Conclusions This new technique uses pretarsal levator aponeurosis to connect levator muscle and upper eyelid skin,it transmits the dynamic power of the levator muscle to the lid skin directly,which created palperbral crease more anatomically and physiologically.In the meantime,levator advancement can be performed to correct blepharoptosis.Furthermore,"skin adhesion" is formed between levator aponeurosis and dermis in a plane planar manner,allowing for more stable palperbralpalpebral crease and more preserved,and preserving orbicularis oculi in situ,leaving no gap in skin and thus reducing eyelid scar formation.

Research on equilibrium solubility and apparent oil/water partition coefficient of flavonoids components in hawthorn leaves by similarity analysis / 中草药

Objective To study the equilibrium solubility and oil/water partition coefficient of Hawthorn leaves flavonoids (HLF) components, and compare their similarity, to lay the foundation for the characterization of the overall water soluble and fat soluble HLF components. Methods Taking HLF components as model drug, rutin, quercetin, and hyperin as representative components. The HPLC method was used to determine the equilibrium solubility and apparent oil/water distribution coefficient (Papp) of the components at different pH values and water. The similarity was evaluated by the vector cosine method (cosines) and Grubbs method (Grubbs). Chromatographic conditions: The chromatographic column was Zorbax Eclipse Plus C18 column (250 mm × 4.6 mm, 5 μm), and the flow phase was acetonitrile (A) - 0.4% phosphate solution (B). The gradient elution program was 0—10 min (80% B), 10—11 min (80%—60% B), 11—20 min (60% B), 20—21 min (60%—80% B), and 21—25 min (80% B); The detection wavelength was 360 nm; The volume flow was 0.8 mL/min; The column temperature was 40 ℃. Results The equilibrium solubility and Papp of rutin, quercetin, hyperin were similar in different pH buffer solution and water. The solubility value of lutin, quercetin, and hyperoside in thebuffer solution of different pH were 0.998, 0.988, and 0.987, respectively. The cosine value of the apparent oil-water distribution coefficient was 0.976, 0.981, and 0.978, respectively. The cosine value was greater than 0.9. The Grubbs value of equilibrium solubility of lutin, quercetin and hyperoside were 1.057, 1.083, 1.124, 1.117, 1.022, 1.030, 1.082, and 1.112, respectively. The Grubbs values of the apparent oil-water distribution coefficient were 1.125, 1.107, 1.079, 1.034, 1.041, 1.037, 1.129, and 1.128. The results of Grubbs were less than G critical value of 1.153, and the similarity was good. Conclusion The similarity analysis reflects that the degree of dispersion of components objectively, which could increase the science of components evaluation and provide the basis for the formulation design of components of traditional Chinese medicine.

Sexual Dysfunction among Chinese Nurses: Prevalence and Predictors / 生物医学与环境科学（英文）

This study aimed to estimate the prevalence of and identify the factors influencing female sexual dysfunction (FSD) among Chinese nurses. A cross-sectional survey was conducted from March 2013 to May 2014 among 6 hospitals in Suzhou, China. In total, 2,030 married female nurses were included in the analysis. Data on the sociodemographic, lifestyle, and self-reported health status of the participants were collected, and the participants were asked to complete the Chinese version of the 19-item Female Sexual Function Index (FSFI) questionnaire. In total, 1,035 (50.99%) participants were found to have FSD. Logistic regression analysis showed that increasing age and higher body mass index, lower salary, and poor/very poor self-reported health status were risk factors for FSD; however, regular social activity and physical exercise were protective factors for FSD. The findings of this study suggest that further interventional studies are warranted to study the sexual health among Chinese nurses in detail.

An analysis on hereditary deafness gene test results of 93 cases of deaf children / 预防医学

Objective To learn the situation of deafness gene among deaf children and to provide suggestions for intervention.Methods Twenty hot spot mutations of the common deafness genes of GJB2,GJB3,MT -RNR1,SLC26A4 for 93 deafness patients were detected by MALDI -TOF -MS,and Sanger sequencing method was used to detect the whole exon of the gene for the heterozygous mutant.Results A total of 48 cases were detected with mutation among the 93 patients using MALDI -TOF -MS,and the detection rate was 51.61%.Thirty five cases were GJB2 mutation,and the detection rate was 37.63%,in which 24 cases were homozygous mutation or compound heterozygous mutations and 11 cases were heterozygous mutation.Thirteen cases were SLC26A4 mutation,and the detection rate was 13.98%,in which 6 cases were homozygous mutation or compound heterozygous mutations and 7 cases were single heterozygous mutation.Mutation in MT -RNR1 and GJB3 gene were not detected.Among the 18 mutation cases,17 cases were detected the whole exon of the gene with mutation using Sanger sequencing,and 12 cases were detected other loci heterozygous mutation (70.59%).And a total of 42 cases were found out the cause of the deafness,and the detection rate was 45.16%.Conclusion The mutation of the common deafness gene in patients with deafness in the region has a high detection rate.The whole exon of the gene with mutation was detected,which can improve the detection rate of the cause of deafness.

Regulating effect of activated NF-κB on edema induced by traumatic brain injury of rats

OBJECTIVE@#To observe the effect of nuclear transcription factor-κB (NF-κB) on cerebral edema in rats with traumatic brain injury (TBI).@*METHODS@#Male SD rats with fluid percussion injury (FPI) were selected. After separation and culture, rats' astrocytes all suffered FPI. The expression of NF-κB and the water content were detected at the animal and cellular levels, while the activity of NOX was evaluated at the cellular level.@*RESULTS@#According to the results, the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI, while NF-κB inhibitor BAY11-7082 could significantly reduce the effect of TBI. 1 and 3 h after FPI of astrocytes, the activation of NF-κB was increased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes. After the injury of astrocytes, the activity of NOX was also increased, while BAY 11-7082 could reduce the activity of NOX.@*CONCLUSIONS@#The results show that the activation of NF-κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.

Regulating effect of activated NF-κB on edema induced by traumatic brain injury of rats

Objective: To observe the effect of nuclear transcription factor-κB (NF-κB) on cerebral edema in rats with traumatic brain injury (TBI). Methods: Male SD rats with fluid percussion injury (FPI) were selected. After separation and culture, rats' astrocytes all suffered FPI. The expression of NF-κB and the water content were detected at the animal and cellular levels, while the activity of NOX was evaluated at the cellular level. Results: According to the results, the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI, while NF-κB inhibitor BAY11-7082 could significantly reduce the effect of TBI. 1 and 3 h after FPI of astrocytes, the activation of NF-κB was increased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes. After the injury of astrocytes, the activity of NOX was also increased, while BAY 11-7082 could reduce the activity of NOX. Conclusions: The results show that the activation of NF-κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.

Clinical implication of histone H2 B monoubiquitination level in primary colonic carcinoma / 临床与实验病理学杂志

Purpose To explore the role of histone H2B monoubiquitination in primary colonic carcinoma and clinicopathological pa-rameters of colonic carcinoma. Methods To detecte the expression of histone H2B monoubiquitination in 116 cases of primary colonic carcinoma and 15 cases of normal colonic mucosal tissue by avidin-biotin-peroxidase complex ( ABC)-immunohistochemistry. Results The level of histone H2B monoubiquitination was significantly reduced in poorly differentiated colonic carcinoma 19. 4% (6/31) compared with that of well-differentiated colonic carcinoma 49. 4%(42/85), moderately differentiated colonic carcinoma 49. 4% (42/85) and normal colonic mucosal tissue 86. 7% (13/15) (P0. 05). Conclusion Histone H2B monoubiquitination is obviously associated with the progression of primary colonic carcinoma. Although its specific mechanism still remains unclear, histone H2B monoubiquitination could be a novel potential molecular marker for early diagnosis, clinic treatment and prognosis evaluation.

Comparison of holmium laser enucleation and plasmakinetic enucleation of the prostate for benign prostatic hyperplasia / 中国综合临床

Objective To evaluate and compare the efficacy of holmium laser enucleation of the prostate(HoLEP) and plasmakineticenucleation of the prostate(PKEP) for treating benign prostatic hyperplasia (BPH).Methods A total of 86 cases of BPH were selected from the Shandong Energy Zibo Mining Group Co.Ltd Central Hospital from January 2013 to June 2014.The patients received either HoLEP (40 cases) or PKEP(46 cases) treatment.Clinical data including postvoid residual volume (PVR),international prostate symptom score(IPSS),quality-of-life score(QOL score),maximum urinary flow rate (MFR) were evaluated before and 3 months after operation.Blood loss in operation,operation time,weight of resected prostate tissue,bladder irrigation time,catheterization time and length of hospital stay were also collected from the perioperative period.Results Three months after surgery,PVR,IPSS,QOL and MFR were all significantly improved in both HoLEP and PKEP groups compared with the data before operation (P =0.000),while there was no significant difference between the two groups(P＞0.05).Patients from the HoLEP group showed markedly lower values of bone loss in operation than that of PKEP group((69.5±23.6) ml vs.(87.5±38.0) ml,P=0.011).There were no significant differences in terms of other indexes (P＞ 0.05).Conclusion HoLEP and PKEP have similar efficiency in treating BPH,while HoLEP is suggested superior in regard of bone loss.

A study on the detection rate of deafness predisposing genes among newborns / 预防医学

Objective To learn the detection rate of deafness predisposing genes among newborns in order to provide suggestions for the prevention of hereditary hearing loss.Methods By means of MALDI -TOF,a total of 4 025 newborns were accepted for newborn hearing and deafness predisposing genetic screening.Four common deafness predisposing genes including GJB2,GJB3,12SrRNA and SLC26A4 were detected,which included 20 hot mutation sites.Results Of the 4 025 subjects,231 were detected with deafness predisposing genes and the positive rate was 5.71%.The total rate of pathogenic mutation was 1.74‰(7 /4 025),including 1 with GJB2 235delC homozygous mutation,1 with GJB2 235delC heterozygous mutation plus 299_300 del AT heterozygous mutation and 5 with 12SrRNA homozygous mutation.The positive rate of single heterozygous mutation was 5.54% (223 /4 025).Fourteen hot mutation sites were detected.GJB2 235delC was the most common one,followed by IVS7 -2A→G.There were 109 cases with GJB2 235delC and 50 cases with IVS7-2A→G.The positive rate was 2.71% and 1.24% respectively,which was 47.19% and 21.65% of the total detection rate respectively.Conclusion The detection rate of deafness predisposing genes is high among newborns.Expanding screening sites could facilitate the detection for the carrier of deafness gene.